Scientists have created a new way to grow and study organelles for cancer research. This was reported by the University of California at Los Angeles.
Organelles are miniature resemblances of human or animal organs that are created from living cells and function like a real counterpart. They can be produced not only from healthy, but also from cancerous cells, to study the course of oncology. By reconstructing patients’ tumors, researchers can test different drugs to see how the cancer will respond to treatment. A major problem with modern organoids is the difficulty of identifying differences between different specimens.
Michael Teitel and colleagues have developed a method to print organoids using a 3D bioprinter by applying cells to a thin layer of helper extracellular protein that leads to the formation of three-dimensional mini-tumors without altering tissue histology and gene expression. To monitor the pattern during the experiment, the authors used the HSLCI method, an imaging system that allows you to measure the weight of living cells in real time. The resulting data is analyzed using machine learning algorithms to capture subtle changes.
“Using this method, we can accurately measure the mass of thousands of organelles at once. This information helps identify which organelles are susceptible or resistant to particular treatments, which can be used to quickly select the most effective treatment options for patients,” explain the authors.
The researchers were able to determine the effects of certain drugs on cells six hours after drug administration. Even in samples consisting of very homogeneous cell lines consisting mainly of cells exposed to treatment, it was also possible to identify small groups of cells that did not respond to drugs.
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