Researchers at Yanmanashi University in Japan cloned mice from freeze-dried (freeze-dried) somatic cells. This is the first successful cloning of its kind. The course of the study is described in an article in the journal. Nature Communication.
Cloning took place in nine stages. To begin with, the scientists restored the lyophilized cells with liquid, but the results of the analyzes showed that the membrane of each of the cells was damaged. To activate the cells, the scientists had to inject the nuclei of the freeze-dried cells into the seedless eggs. Then came the cell culture process, which showed that, 96 hours later, the DNA of all embryos still remained damaged.
In order not to risk the experiment, the biotechnologists decided not to inject the resulting material into the uterus of a laboratory mouse, but to extract embryonic stem cells for further maturation. When the embryos reached the bicellular phase, they were injected into female mouse oocytes. After 19.5 days, the fertilized mice gave birth by cesarean section.
According to experts, the success rate of cloning from lyophilized somatic cells is 0.02% – significantly lower than the legendary sheep Dolly with a coefficient of 0.4%.
Source: Gazeta
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